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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant job in advertising and marketing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be explained. Two extra splice variants were being described but in depth nucleotide sequence information and facts will not be but accessible.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged via the antibody.
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This guide outlines further more doable optimization of mobile and immunoassay parameters to ensure the very best final results are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining with the blocked antibody vs . the antibody alone, one can see which staining is specific.
Concentrate on Information get more info and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which contribute to your temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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